The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.
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PolyATtract® System 1000 with Magnetic Separation Stand Z5420 Each system contains a Magnetic Separation Stand and sufficient reagents for the isolation of RNA from up to 2 grams of tissue or 4 × 10 8 cultured cells.
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streptavidin coupled to paramagnetic particles and a magnetic separation stand (Figure 1). The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free deionized water. This procedure yields an essentially pure fraction of mature mRNA after only a single round of magnetic separation. Figure 1.
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The MagneSphere® Magnetic Separation Stands can be used in conjunction with the SA-PMPs and any of the PolyATtract ® Systems. The MagneSphere ® Stands use the same samarium/cobalt magnet used in the PolyATtract ®
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1. Antibody purification using Magne™ Protein A Beads or Magne™ Protein G Beads requires a magnetic stand. The beads can be used with a variety of magnetic stands available from Promega (see Section 7) to purify antibody from 1–96 samples in parallel, with sample volumes ranging from 20µl to 30ml. 2.
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Add DNA IQ™ Resin, and wash your sample using a magnetic stand. Thirty minutes later, you have purified DNA. Some samples, such as tissue and hair, require pretreatment with Proteinase K.
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• Magnetic separation stand: For individual tubes, we recommend the MagneSphere® Technology Magnetic Separation Stand (Promega Cat.# Z5342). For samples in 96-well plate format, we recommend Life Technologies DynaMag™-96 Side (Part# 12331D) • Absolute ethanol (200 proof, molecular biology grade) • Nuclease-free water
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The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.
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• magnetic separation stand (see note below) • NANOpure® or double-distilled water Note: This protocol requires a magnetic separation stand. The MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342) can be used to hold one to twelve 1.5ml microcentrifuge tubes. The MagneSphere®
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The MagneGST™ Pull-Down System(a–c) provides GSH-linked magnetic particles that allow simple immobilization of GST-fusion bait proteins from bacterial lysates and an in vitro transcription/translation system for expression of prey proteins. Prey proteins are isolated using the appropriate bait protein attached to the MagneGST™ (GSH ...
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نشانی: شماره 1688، جاده شرقی گاوک، منطقه جدید پودونگ، شانگهای، چین.
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